Molecular Identification of Pentachlorophenol (PCP) Tolerant Bacterial Communities in Contaminated Groundwater

Author(s): Stokes, C. ;  Prewitt, M.;  Borazjani, H.

Pentachlorophenol (PCP), a highly toxic and recalcitrant wood preservative, contaminates groundwater aquifers in many areas of United States. Improper handling, storage, and disposal practices in the past have led to the contamination of groundwater at many wood treatment facilities. Biosparging, the injection of clean air and nutrients under pressure into the groundwater system, has emerged as a viable in-situ treatment option for removal of this type of contamination. Previous studies in this area have relied on growth media cultures for isolation and identification of the bacterial community that is responsible for the degradation of the pollutant. However, molecular identification of DNA extracted from the contaminated groundwater will provide a more accurate description of the microbial community. Seven biosparging wells located at a wood treatment facility with a PCP groundwater contamination in central Mississippi have been monitored since 2001. Groundwater samples from these existing wells were taken quarterly and examined for total PCP concentration. DNA was extracted from these water samples using a WaterMaster DNA purification kit. The 16s region from this DNA was also amplified using bacterial specific primers and then cloned into E. coli cells. Cloned E. coli cells were extracted and sequenced for identification. The goals of this research were to identify the most PCP-tolerant bacterial communities and to determine the PCP tolerance limits of these bacterial communities.


Technical Presentations

  • Delta Water Quality
  • Delta Water and Agriculture
  • Wetlands
  • Water Quality
  • Sediments
  • Non-Point
  • Management and Sustainability
  • Wood Treatment
  • Modeling
  • Soil and Water Treatment


Responsible Site Design: Implementing Innovative Stormwater Management Strategies

The primary goal of the workshop is to create a dynamic learning experience that examines the role of stormwater management in the built environment. The workshop will focus on integrating ecologically sound water management approaches into site design. After the workshop, attendees will be familiar with the following concepts and technical issues:

  • Knowledge of the stormwater treatment chain
  • Knowledge of the impact of land use codes on stormwater management
  • Application of a design process that mitigates the effects of stormwater on-site
  • Knowledge of the relationship between land use codes and design for innovative stormwater management


For information contact:
Jessie Schmidt
Box 9680
Mississippi State, MS 39762